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elisa kits  (R&D Systems)


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    R&D Systems elisa kits
    Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
    Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kits/product/R&D Systems
    Average 93 stars, based on 12 article reviews
    elisa kits - by Bioz Stars, 2026-02
    93/100 stars

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    1) Product Images from "Pubertal exposure to dietary advanced glycation end products disrupts ductal morphogenesis and induces atypical hyperplasia in the mammary gland."

    Article Title: Pubertal exposure to dietary advanced glycation end products disrupts ductal morphogenesis and induces atypical hyperplasia in the mammary gland.

    Journal: Breast cancer research : BCR

    doi: 10.1186/s13058-023-01714-4

    Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in a RAGE dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B ELISA (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
    Figure Legend Snippet: Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in a RAGE dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B ELISA (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001

    Techniques Used: Migration, Enzyme-linked Immunosorbent Assay, Isolation, Co-culture Assay, Transwell Migration Assay, Cell Culture, Ex Vivo



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    Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
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    Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
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    Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
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    Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in a RAGE dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B ELISA (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001

    Journal: Breast cancer research : BCR

    Article Title: Pubertal exposure to dietary advanced glycation end products disrupts ductal morphogenesis and induces atypical hyperplasia in the mammary gland.

    doi: 10.1186/s13058-023-01714-4

    Figure Lengend Snippet: Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in a RAGE dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B ELISA (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001

    Article Snippet: Mouse RAGE levels were determined using ELISA kits from R&D Systems (Minneapolis, MN; #DY1179) and performed as per the manufacturers’ instructions.

    Techniques: Migration, Enzyme-linked Immunosorbent Assay, Isolation, Co-culture Assay, Transwell Migration Assay, Cell Culture, Ex Vivo